MSE PRO Rat GPR131(G Protein Coupled Receptor 131) ELISA Kit
SKU: EK0064877-96T
MSE PRO™ Rat GPR131(G Protein Coupled Receptor 131) ELISA Kit
Specifications:
| Product name | Rat GPR131(G Protein Coupled Receptor 131) ELISA Kit |
| Reactivity | Rat |
| Alternative Names | GPBAR1; GPCR; BG37; GPCR19; M-BAR; TGR5; G Protein-Coupled Bile Acid Receptor 1; G-protein coupled receptor GPCR19; Membrane-type receptor for bile acids |
| Assay Type | Sandwich |
| Sensitivity | 0.11 ng/mL |
| Standard | 40 ng/mL |
| Detection Range | 0.63-40 ng/mL |
| Sample Type | Serum, plasma and other biological fluids. |
| Assay Length | 3.5h |
| Research Area | Neuro science |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GPR131. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GPR131. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GPR131, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GPR131 in the samples is then determined by comparing the OD of the samples to the standard curve. |