MSE PRO Rat ACV-A(Activin A) ELISA Kit
SKU: EK0064638-96T
MSE PRO™ Rat ACV-A(Activin A) ELISA Kit
Specifications:
| Product name | Rat ACV-A(Activin A) ELISA Kit |
| Reactivity | Rat |
| Alternative Names | ACVA; Activin Beta A Beta A Homodimer |
| Assay Type | Sandwich |
| Sensitivity | 4.57 pg/mL |
| Standard | 1000 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Endocrinology; Reproductive science; Hormone metabolism |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ACV-A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ACV-A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ACV-A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ACV-A in the samples is then determined by comparing the OD of the samples to the standard curve. |