MSE PRO Pig TAT(Thrombin/Antithrombin Complex) ELISA Kit
SKU: EK0064522-96T
MSE PRO™ Pig TAT(Thrombin/Antithrombin Complex) ELISA Kit
Specifications:
| Product name | Pig TAT(Thrombin/Antithrombin Complex) ELISA Kit |
| Reactivity | Pig |
| Alternative Names | Thrombin/Antithrombin Complex |
| Assay Type | Sandwich |
| Sensitivity | 21.7 pg/mL |
| Standard | 4000 pg/mL |
| Detection Range | 62.5-4000 pg/mL |
| Sample Type | Plasma |
| Assay Length | 3.5h |
| Research Area | Signal transduction; Enzyme & Kinase; Metabolic pathway; Hematology |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig TAT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig TAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig TAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig TAT in the samples is then determined by comparing the OD of the samples to the standard curve. |