MSE PRO NT(Nitrotyrosine) ELISA Kit
SKU: EK0064616-96T
MSE PRO™ NT(Nitrotyrosine) ELISA Kit
Specifications:
| Product name | NT(Nitrotyrosine) ELISA Kit |
| Reactivity | General |
| Alternative Names | Nitrotyrosine |
| Assay Type | Competitive Inhibition |
| Sensitivity | 2.1 ng/mL |
| Standard | 500 ng/mL |
| Detection Range | 7.82-500 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 2h |
| Research Area | Metabolic pathway; Infection immunity; Rheumatology; |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NT in the samples is then determined by comparing the OD of the samples to the standard curve. |