MSE PRO Mouse NFκB-p65 (Nuclear Factor Kappa B p65) ELISA Kit
SKU: EK0064336-48T
MSE PRO™ Mouse NFκB-p65 (Nuclear Factor Kappa B p65) ELISA Kit
Specifications:
| Product name | Mouse NFκB-p65 (Nuclear Factor Kappa B p65) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | NF kappa B p65delta3; NFKB3 |
| Assay Type | Sandwich |
| Sensitivity | 12.2 pg/mL |
| Standard | 2000 pg/mL |
| Detection Range | 31.25-2000 pg/mL |
| Sample type | serum, plasma, tissue homogenates |
| Assay length | 3.5h |
| Research Area | Immunology |
| Uniprot ID | Q04207 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve. |