MSE PRO Mouse LAMC2(Laminin Gamma 2) ELISA Kit
SKU: EK0064507-96T
MSE PRO™ Mouse LAMC2(Laminin Gamma 2) ELISA Kit
Specifications:
| Product name | Mouse LAMC2(Laminin Gamma 2) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | B2T; EBR2; EBR2A; LAMB2T; LAMNB2; Kalinin/nicein/epiligrin 100 kDa subunit; Cell-scattering factor 140 kDa subunit; Ladsin 140 kDa; Large adhesive scatter factor 140 kDa |
| Assay Type | Sandwich |
| Sensitivity | 6.7 pg/mL |
| Standard | 1000 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Developmental science |
| Uniprot ID | Q61092 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LAMC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LAMC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LAMC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LAMC2 in the samples is then determined by comparing the OD of the samples to the standard curve. |