MSE PRO MDA(Malondialdehyde) ELISA Kit
SKU: EK0064273-48T
MSE PRO™ MDA (Malondialdehyde) ELISA Kit
Specifications:
| Product name | MDA(Malondialdehyde) ELISA Kit |
| Reactivity | General |
| Alternative Names | MDA |
| Assay Type | Competitive Inhibition |
| Sensitivity | 9.47 ng/mL |
| Standard | 2000 ng/mL |
| Detection Range | 31.25-2000 ng/mL |
| Sample type | serum, plasma and other biological fluids |
| Assay length | 2h |
| Research Area | Metabolic pathway; Hepatology; Hormone metabolism |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with MDA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MDA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MDA in the samples is then determined by comparing the OD of the samples to the standard curve. |