MSE PRO Human MEGF8(Multiple Epidermal Growth Factor-Like Domains Protein 8) ELISA Kit
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MSE PRO™ Human MEGF8(Multiple Epidermal Growth Factor-Like Domains Protein 8) ELISA Kit
Specifications:
| Product name | Human MEGF8(Multiple Epidermal Growth Factor-Like Domains Protein 8) ELISA Kit |
| Reactivity | Human |
| Alternative Names | C19orf49; CRPT2; EGFL4; SBP1 |
| Assay Type | Sandwich |
| Sensitivity | 0.063 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | serum, plasma, saliva, tears and other biological fluids and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Signal transduction |
| Uniprot ID | Q7Z7M0 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MEGF8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MEGF8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MEGF8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MEGF8 in the samples is then determined by comparing the OD of the samples to the standard curve. |