MSE PRO Human KL-6(Krebs Von den Lungen-6) ELISA Kit
SKU: EK0064641-96T
MSE PRO™ Human KL-6(Krebs Von den Lungen-6) ELISA Kit
Specifications:
| Product name | Human KL-6(Krebs Von den Lungen-6) ELISA Kit |
| Reactivity | Human |
| Alternative Names | KL-6 |
| Assay Type | Sandwich |
| Sensitivity | 14.21 U/mL |
| Standard | 2000 U/mL |
| Detection Range | 31.25-2000 U/mL |
| Sample Type | serum, plasma, tissue homogenates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Interstitial lung disease |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KL-6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KL-6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KL-6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KL-6 in the samples is then determined by comparing the OD of the samples to the standard curve. |