MSE PRO Human ET-1(Endothelin1) ELISA Kit
SKU: EK0064293-48T
MSE PRO™ Human ET-1 (Endothelin1) ELISA Kit
Specifications:
| Product name | Human ET-1 (Endothelin1) ELISA Kit |
| Reactivity | Human |
| Alternative Names | ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1 |
| Assay Type | Sandwich |
| Sensitivity | 2.73 pg/mL |
| Standard | 500 pg/mL |
| Detection Range | 7.82-500 pg/mL |
| Sample type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay length | 3.5h |
| Research Area | Endocrinology; Cardiovascular biology |
| Uniprot ID | P05305 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve. |