MSE PRO Human CA15-3(Carbonhydrate Antigen 15-3) ELISA Kit
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MSE PRO™ Human CA15-3(Carbonhydrate Antigen 15-3) ELISA Kit
Specifications:
| Product name | Human CA15-3(Carbonhydrate Antigen 15-3) ELISA Kit |
| Reactivity | Human |
| Alternative Names | CA15-3 |
| Assay Type | Sandwich |
| Sensitivity | 0.47 U/mL |
| Standard | 100 U/mL |
| Detection Range | 1.57-100 U/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Tumor immunity; Infection immunity |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA15-3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA15-3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA15-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA15-3 in the samples is then determined by comparing the OD of the samples to the standard curve. |