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MSE PRO Human Ab1-40(Amyloid Beta Peptide 1-40) ELISA Kit

SKU: EK0064281-48T

  • $ 31995
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MSE PRO™ Human Ab1-40 (Amyloid Beta Peptide 1-40) ELISA Kit

Specifications:

Product name Human Ab1-40 (Amyloid Beta Peptide 1-40) ELISA Kit
Reactivity Human
Alternative Names Amyloid Beta Peptide 1-40
Assay Type Sandwich
Sensitivity 4.48 pg/mL
Standard 1000 pg/mL
Detection Range 15.63-1000 pg/mL
Sample type Serum, plasma and other biological fluids
Assay length 3.5h
Research Area Infection immunity; Neuro science
Test principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ab1-40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Ab1-40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Ab1-40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Ab1-40 in the samples is then determined by comparing the OD of the samples to the standard curve.