MSE PRO Horse 5-HT(5-Hydroxytryptamine) ELISA Kit
SKU: EK0064825-96T
MSE PRO™ Horse 5-HT(5-Hydroxytryptamine) ELISA Kit
Specifications:
| Product name | Horse 5-HT(5-Hydroxytryptamine) ELISA Kit |
| Reactivity | Horse |
| Alternative Names | 5HT; Serotonin; N-Methyl-Gamma |
| Assay Type | Competitive Inhibition |
| Sensitivity | 5.85 ng/mL |
| Standard | 900 ng/mL |
| Detection Range | 14.07-900 ng/mL |
| Sample Type | serum, plasma |
| Assay Length | 2.5h |
| Research Area | Signal transduction; Infection immunity; Neuro science; Gastroenterology |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse 5-HT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve. |