MSE PRO Goat MPO(Myeloperoxidase) ELISA Kit
SKU: EK0064491-96T
MSE PRO™ Goat MPO(Myeloperoxidase) ELISA Kit
Specifications:
| Product name | Goat MPO(Myeloperoxidase) ELISA Kit |
| Reactivity | Goat |
| Alternative Names | Mpo; Myeloperoxidase |
| Assay Type | Sandwich |
| Sensitivity | 0.65 ng/mL |
| Standard | 100 ng/mL |
| Detection Range | 1.57-100 ng/mL |
| Sample Type | Serum, plasma and other biological fluids. |
| Assay Length | 3.5h |
| Research Area | Enzyme & Kinase; Infection immunity; Cardiovascular biology; Hepatology |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat MPO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat MPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat MPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat MPO in the samples is then determined by comparing the OD of the samples to the standard curve. |