MSE PRO EasyStep Mouse PG(Progesterone) ELISA Kit
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MSE PRO™ EasyStep Mouse PG(Progesterone) ELISA Kit
Specifications:
| Product name | EasyStep Mouse PG(Progesterone) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | P4; Pregn-4-Ene-3,20-Dione |
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.7 ng/mL |
| Standard | 100 ng/mL |
| Detection Range | 1.56-100 ng/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Endocrinology; Reproductive science; Hormone metabolism; |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse PG antigen, and the Mouse PG standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse PG to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse PG in the samples is then determined by comparing the OD of the samples to the standard curve. |