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MSE PRO EasyStep Human SAA(Serum Amyloid A) ELISA Kit

SKU: EK0064866-96T

  • $ 58795
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MSE PRO™ EasyStep Human SAA(Serum Amyloid A) ELISA Kit

Specifications:

Product name EasyStep Human SAA(Serum Amyloid A) ELISA Kit
Reactivity Human
Alternative Names SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein
Assay Type Sandwich
Sensitivity 0.15 ng/mL
Standard 50 ng/mL
Detection Range 0.78-50 ng/mL
Sample Type Serum, plasma
Assay Length 1.5h
Research Area Infection immunity; Kidney biomarker; Hepatology; Urology
Uniprot ID P0DJI8
Test principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SAA, and the Human SAA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human SAA. After TMB substrate solution is added, only those wells that contain Human SAA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SAA in the samples is then determined by comparing the OD of the samples to the standard curve.