MSE PRO EasyStep Human GH(Growth Hormone) ELISA Kit
SKU: EK0064527-96T
MSE PRO™ EasyStep Human GH(Growth Hormone) ELISA Kit
Specifications:
| Product name | EasyStep Human GH(Growth Hormone) ELISA Kit |
| Reactivity | Human |
| Alternative Names | GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal |
| Assay Type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Standard | 800 pg/mL |
| Detection Range | 12.5-800 pg/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Endocrinology; Neuro science; Hormone metabolism |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GH, and the Human GH standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human GH. After TMB substrate solution is added, only those wells that contain Human GH and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GH in the samples is then determined by comparing the OD of the samples to the standard curve. |