MSE PRO EasyStep Human CRP(C Reactive Protein) ELISA Kit
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MSE PRO™ EasyStep Human CRP(C Reactive Protein) ELISA Kit
Specifications:
| Product name | EasyStep Human CRP(C Reactive Protein) ELISA Kit |
| Reactivity | Human |
| Alternative Names | C-RP; PTX1; Pentraxin-Related; Pentraxin 1 |
| Assay Type | Sandwich |
| Sensitivity | 53 pg/mL |
| Standard | 15 ng/mL |
| Detection Range | 0.23-15 ng/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Tumor immunity; Infection immunity; Cardiovascular biology; Rheumatology |
| Uniprot ID | P02741 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CRP, and the Human CRP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human CRP. After TMB substrate solution is added, only those wells that contain Human CRP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRP in the samples is then determined by comparing the OD of the samples to the standard curve. |