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MSE PRO EasyStep Human ADP(Adiponectin) ELISA Kit

SKU: EK0064739-96T

  • $ 45795
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MSE PRO™ EasyStep Human ADP(Adiponectin) ELISA Kit

Specifications:

Product name EasyStep Human ADP(Adiponectin) ELISA Kit
Reactivity Human
Alternative Names GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1
Assay Type Sandwich
Sensitivity 1.9 ng/mL
Standard 200 ng/mL
Detection Range 3.13-200 ng/mL
Sample Type Serum, plasma
Assay Length 1.5h
Research Area Metabolic pathway; Infection immunity; Cardiovascular biology
Uniprot ID Q15848
Test principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ADP, and the Human ADP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human ADP. After TMB substrate solution is added, only those wells that contain Human ADP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ADP in the samples is then determined by comparing the OD of the samples to the standard curve.