MSE PRO Dog β2-MG(Beta-2-Microglobulin) ELISA Kit
SKU: EK0064777-96T
MSE PRO™ Dog β2-MG(Beta-2-Microglobulin) ELISA Kit
Specifications:
| Product name | Dog β2-MG(Beta-2-Microglobulin) ELISA Kit |
| Reactivity | Dog |
| Alternative Names | Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin |
| Assay Type | Sandwich |
| Sensitivity | 0.23 µg/mL |
| Standard | 50 µg/mL |
| Detection Range | 0.78-50 µg/mL |
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids. |
| Assay Length | 3.5h |
| Research Area | Infection immunity; Kidney biomarker |
| Uniprot ID | P19341 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog β2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog β2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog β2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog β2-MG in the samples is then determined by comparing the OD of the samples to the standard curve. |