MSE PRO Cattle PSPB(Pregnancy Specific Protein B) ELISA Kit
SKU: EK0064909-96T
MSE PRO™ Cattle PSPB(Pregnancy Specific Protein B) ELISA Kit
Specifications:
| Product name | Cattle PSPB(Pregnancy Specific Protein B) ELISA Kit |
| Reactivity | Cattle |
| Alternative Names | Pregnancy-associated glycoprotein 1; PAG 1; EC 3.4.23.; Pregnancy-specific protein B; PSP-B |
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.29 ng/mL |
| Standard | 50 ng/mL |
| Detection Range | 0.78-50 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 2.5h |
| Research Area | Signal transduction; Cardiovascular; Cancer; Metabolism |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle PSPB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle PSPB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle PSPB in the samples is then determined by comparing the OD of the samples to the standard curve. |