Lactate Dehydrogenase (LDH) Cytotoxicity Colorimetric Assay Kit
To better serve you, we would like to discuss your specific requirement, Please Contact Us for a quote.
Lactate Dehydrogenase (LDH) Cytotoxicity Colorimetric Assay Kit
| SKU # | E-BC-K771-M |
| Detection Instrument | Microplate reader (450 nm) |
| Detection method: | Colorimetric method |
Product Details
Properties
| Synonyms | LDH |
| Sample type | Cells |
| Detection method |
Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 30 min |
| Other instruments required | Microplate centrifuge, Micropipettor, Water bath |
| Storage | -20℃ |
| Valid period | 12 months |
Images
Q Li Lee et al investigate the function of outer-membrane-acting peptides in killing Gram-negative pathogens. Cytotoxicity on human cell was determined using lactate dehydrogenase (LDH) cytotoxicity colorimetric assay kit (E-BC-K771-M).
Neither vancomycin nor D-11 affects the viability of human HEK-293 cells.
Detection Principle
Lactate dehydrogenase catalyzes the reaction of lactic acid with NAD+ to produce pyruvic acid and NADH. NADH, under the action of PMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, LDH activity can be quantified by measure the OD value at 450 nm.
Kit Components & Storage
| Item | Component | Size 1(96 T) |
Size 2 (500 Assays) |
Storage |
| Reagent 1 | Lysis Solution | 2 mL × 1 vial | 10 mL × 1 vial | -20℃, 12 months |
| Reagent 2 | Substrate | 1.5 mL ×2 vials | 15 mL ×1 vial - | -20℃, 12 months |
| Reagent 3 | Chromogenic Agent | 1.5 mL ×2 vials | 15 mL ×1 vial - | -20℃, 12 months, shading light |
| Reagent 4 | Enzyme Reagent | Powder × 1 vial | Powder × 5 vials | -20℃, 12 months |
| Reagent 5 | Stop Solution | 1.5 mL ×2 vials | 15 mL ×1 vial | -20℃, 12 months |
| Microplate | 96 wells | |||
| Plate Sealer | 2 pieces |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.