(KO Validated) p53 Polyclonal Antibody
SKU: E-AB-93317-200
(KO Validated) p53 Polyclonal Antibody
| SKU # | E-AB-93317 |
| Reactivity | Human |
| Host | Rabbit |
| Applications | WB, IHC, IF, IP |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC, IF, IP |
| Clonality | Polyclonal |
| Immunogen | Recombinant fusion protein of human p53 |
| Abbre | p53 |
| Synonyms | BCC7, LFS1, P53, TRP53, tumor protein p53, TP53 |
| Swissprot | |
| Calculated MW | 23-43 kDa |
| Observed MW | 53 kDa |
| Cellular Localization | Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cell Biology, Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
| IHC | 1:50-1:200 |
| IF | 1:50-1:200 |
| IP | 1:50-1:200 |
Background
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).