IP/CoIP Kit (Agarose)
SKU: EA-IP-K007
IP/CoIP Kit (Agarose)
Background
This product is made of high quality Protein A/G protein covalently bound with agarose gel and can be used for immunoprecipitation (IP) and co-immunoprecipitation (Co-IP). This product has the characteristics of high binding capacity of protein, fast and convenient operation, strong specificity, low non-specific adsorption and wide binding range.
Performance
1. Scope of application
Immunoprecipitation (co-precipitation) of IgG proteins (including most IgG subtypes) from samples of cell lysates, cell secretory supernatants, serums, animal ascites, etc. (See Annex)
2. Binding properties
High purity recombinant Protein A/G protein.
3. Gel properties
Agarose gel granules, average size 50μm.
4. Binding capacity
1mL Sepharose 4B agarose granules are covalently bound to 20mg recombinant Protein A/G protein.
5. Components
0.5 mL of Protein A/G agarose gel stored in 1.5 mL PBS containing preservatives and 50% glycerol.
Product Components
|
Cat. No. |
Component |
Code |
Specification |
Storage |
|
E-IR-IP004 |
Lysis buffer |
L1 |
30 mL |
4°C, 12 months |
|
E-IR-IP001 |
Centrifugal column |
C |
50 piece |
Room temperature, 12 months |
|
EA-IP-007 |
Protein A/G Affinity Agarose |
G1 |
2 mL |
-20°C, 12 months |
|
E-IR-IP006 |
Acid elution buffer |
E3 |
1 mL |
4°C, 12 months |
|
E-BC-R187 |
PBS Buffer, pH7.4 (10×) |
P10 |
50 mL |
4°C, 12 months |
|
E-IR-R310 |
PBST Buffer, pH7.4 (10×) |
P10T |
50 mL |
4°C, 12 months |
|
Manual |
one copy |
|||
Storage
-20°C, 12 months.