His-Tag Polyclonal Antibody Reactivity All
SKU: E-AB-40506-200
His-Tag Polyclonal Antibody Reactivity All
| SKU # | E-AB-40506 |
| Reactivity | All |
| Host | Rabbit |
| Applications | WB |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | All |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide corresponding to 6His tag conjugated to keyhole limpet haemocyanin. |
| Abbre | His-Tag |
| Synonyms | HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6His epitope tag, His tag |
| Calculated MW | 28 kDa |
| Observed MW | 28 kDa |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:1000-2000 |
Background
The His-tag (also called 6xHis-tag) is one of the simplest and most widely used purification tags, with six or more consecutive histidine residues. It is the most widely used protein affinity tag. Due to its small size of only six amino acids, it rarely interferes with the structure or function of the target protein. The His-tag binds strongly to divalent metal ions such as nickel or cobalt, which are immobilized on a resin, allowing for efficient purification through immobilized metal affinity chromatography (IMAC). This method enables rapid and specific isolation of recombinant proteins from complex mixtures, making it a valuable tool in molecular biology and biochemistry.