His-Tag Monoclonal Antibody Host Mouse
SKU: E-AB-48003-200
His-Tag Monoclonal Antibody Host Mouse
| SKU # | E-AB-48003 |
| Reactivity | All |
| Host | Mouse |
| Applications | WB, IP, FCM |
Product Details
| Isotype | IgG2a |
| Host | Mouse |
| Reactivity | All |
| Applications | WB, IP, FCM |
| Clonality | Monoclonal |
| Immunogen | Synthetic peptide corresponding to 6His tag conjugated to keyhole limpet haemocyanin. |
| Abbre | His-Tag |
| Synonyms | HHHHHH epitope tag, HHHHHH tag, Hexa His tag, His tag, Polyhistidine Tag, 6 His epitope tag |
| Concentration | 1 mg/mL |
| Buffer | PBS, pH 7.2. Contains 0.05% proclin 300. |
| Purification Method | Protein A/G Purification |
| Clone No. | 1A5 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:1000-2000 |
| IP | 3μg/sample |
| FCM | 0.2μg |
Background
The 6xHis tag, also known as polyhistidine tag, His6 tag and/or hexa histidine tag, is an amino acid motif consisting of at least 6 histidine residues fused to the carboxyl (C-) or amino (N-) terminus of a target protein in transfected cells. It is one of the simplest and most widely used purification tags in the world.