HIMEDIA Tryptose Serum Broth Base(Modified Newings)
SKU: M2019-500G
HIMEDIA Tryptose Serum Broth Base (Modified Newings) 500G
Intended Use:
Recommended for routine identification of Mycoplasma species.
Composition**
|
Ingredients |
g / L |
|
Tryptose |
20.000 |
|
Sodium chloride |
5.000 |
|
Disodium hydrogen phosphate |
2.500 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 27.50 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Aseptically add the rehydrated contents of one vial of PDTY Selective Supplement (FD334) and 130 ml of pig serum (RM10415) (Inactivate at 56°C for not more than 30 minutes). Mix well and distribute into sterile tubes or flasks as desired.