HIMEDIA RPMI- 1640 w/ L-Glutamine and Sodium bicarbonate w/o Glucose and HEPES
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HIMEDIA RPMI- 1640 w/ L-Glutamine and Sodium Bicarbonate w/o Glucose and HEPES 500ML
Product Description:
Roswell Park Memorial Institute (RPMI) media are a series of media developed by Moore et al for the culture of human normal and neoplastic cells in vitro. RPMI-1640 is the most commonly used medium in the series. A modification of McCoy's 5A medium, the medium was specifically designed to support the growth of human lymphoblastoid cells in suspension culture. Presently the medium is extensively used for a wide range of anchorage dependant cell lines. The medium needs to be supplemented with 5-20% fetal bovine serum. The medium is also known to support growth of cells in the absence of serum.
AL150A is RPMI-1640 medium supplemented with L-glutamine and sodium bicarbonate. It does not contain glucose and HEPES. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
Composition:
|
Ingredients |
mg/L |
|
INORGANIC SALTS |
|
|
Calcium nitrate tetrahydrate |
100.000 |
|
Magnesium sulphate anhydrous |
48.840 |
|
Potassium chloride |
400.000 |
|
Sodium bicarbonate |
2000.000 |
|
Sodium chloride |
6000.000 |
|
Sodium phosphate dibasic anhydrous |
800.000 |
|
AMINO ACIDS |
|
|
Glycine |
10.000 |
|
L-Arginine hydrochloride |
241.000 |
|
L-Asparagine monohydrate |
50.000 |
|
L-Aspartic acid |
20.000 |
|
L-Cystine dihydrochloride |
65.150 |
|
L-Glutamic acid |
20.000 |
|
L-Glutamine |
300.000 |
|
L-Histidine hydrochloride monohydrate |
20.960 |
|
L-Hydroxyproline |
20.000 |
|
L-Isoleucine |
50.000 |
|
L-Leucine |
50.000 |
|
L-Lysine hydrochloride |
40.000 |
|
L-Methionine |
15.000 |
|
L-Phenylalanine |
15.000 |
|
L-Proline |
20.000 |
|
L-Serine |
30.000 |
|
L-Threonine |
20.000 |
|
L-Tryptophan |
5.000 |
|
L-Tyrosine sodium salt dihydrate |
28.830 |
|
L-Valine |
20.000 |
|
VITAMINS |
|
|
Choline chloride |
3.000 |
|
D-Biotin |
0.200 |
|
D-Ca-Pantothenate |
0.250 |
|
Folic acid |
1.000 |
|
Niacinamide |
1.000 |
|
Pyridoxine hydrochloride |
1.000 |
|
Riboflavin |
0.200 |
|
Thiamine hydrochloride |
1.000 |
|
Vitamin B12 |
0.005 |
|
i-Inositol |
35.000 |
|
p-Amino benzoic acid (PABA) |
1.000 |
|
OTHERS |
|
|
Glutathione reduced |
1.000 |
|
Phenol red sodium salt |
5.300 |