HIMEDIA Loeffler Serum Medium Base

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SKU: M1189-100G
Regular price $ 49.95

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HIMEDIA Loeffler Serum Medium Base 100G

Intended Use:

Recommended for the cultivation of Corynebacterium diphtheriae.

Composition**

Ingredients

g / L

HMH infusion from #

0.720

Peptone

0.710

Sodium chloride

0.360

Dextrose (Glucose)

0.710

Egg powder

7.500

Final pH ( at 25°C)

7.6±0.2

 

**Formula adjusted, standardized to suit performance parameters
# Equivalent to Heart muscle, infusion from

Directions

Suspend 10.0 grams in 250 ml purified / distilled water at a temperature of 45°C. Add 750 ml of sterile Bovine serum, mix well and dispense into tubes. Coagulate and sterilize by inspissation for 15 minutes at 80 to 90°C or steaming at 100°C for 10-15 minutes.