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Formate Colorimetric Assay Kit– MSE Supplies LLC

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Formate Colorimetric Assay Kit

SKU: E-BC-K1100-M-96

  • $ 34095
  • Save $ 6000



Formate Colorimetric Assay Kit

SKU # E-BC-K1100-M
Detection Instrument Microplate reader (450 nm)
Detection Method Colorimetric method


Product Details

Properties

Sample Type Serum, plasma, animal tissue
Sensitivity 8.20 μmol/L
Detection Range 8.20-800 μmol/L
Detection Method Colorimetric method
Assay type Quantitative
Assay time 70 min
Precision Average inter-assay CV: 3.900% | Average intra-assay CV: 3.300%
Other instruments required Pipettor, Water bath, Centrifuge
Storage -20℃
Valid period 12 months

 

Dilution of Sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (8.20-800 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human serum 1
Dog serum 1
Mouse serum 1
Horse serum 1
Porcine serum 1
Rat plasma 1
10% Rat liver tissue homogenate 1
10% Rat kidney tissue homogenate 1
10% Rat spleen tissue homogenate 1
10% Rat brain tissue homogenate 1

 

Note: The diluent is reagent 1.

 

Detection Principle

Formic acid is the simplest carboxylic acid, whose chemical formula is CH2O2. It is often used as an antimicrobial/preservative in livestock feed. Formic acid can block some of the decay processes in feed, making the nutritional value of feed maintain longer. Under normal circumstances, the physiological concentration of formic acid is low and easy to metabolize, but in the case of methanol poisoning, the concentration can reach 5 mmoL/L. Similarly, long-term exposure to excessive levels of formaldehyde can also increase the content of formic acid in blood and urine.

 

Kit Components & Storage

Item Component Size (96 T) Storage
Reagent 1 Extracting Solution 60 mL × 2 vials -20℃, 12 months
Reagent 2 Substrate A Powder × 2 vials -20℃, 12 months, shading light
Reagent 3 Substrate B Powder × 1 vial -20℃, 12 months, shading light
Reagent 4 Chromogenic Agent 1.5 mL × 2 vials -20℃, 12 months, shading light
Reagent 5 10 mmol/L Standard 1.0 mL × 1 vial -20℃, 12 months
Microplate 96 wells No requirement
Plate Sealer 2 pieces

 

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

 

Technical Data:

Parameter:

Intra-assay Precision

Three human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).

Parameters Sample 1 Sample 2 Sample 3
Mean (μmol/L) 16.50 135.00 264.00
%CV 3.6 3.2 3.1


Inter-assay Precision

Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.

Parameters Sample 1 Sample 2 Sample 3
Mean (μmol/L) 16.50 135.00 264.00
%CV 4.1 3.8 3.8

 

Recovery

Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 100%.


Standard 1 Standard 2 Standard 3
Expected Conc. (μmol/L) 125 362 554
Observed Conc. (μmol/L) 126.3 358.4 554.0
Recovery rate (%) 101 99 100


Sensitivity

The analytical sensitivity of the assay is 8.2 μmol/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.

 

Standard Curve

As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:

Concentration (mmol/L) 0 100 200 300 400 500 600 800
Average OD 0.154 0.327 0.492 0.677 0.838 1.025 1.171 1.593
Absoluted OD 0.000 0.174 0.338 0.524 0.684 0.872 1.017 1.440