Bioneer Taq DNA Polymerase, 10X Reaction Buffer without MgCl2, 20 mM MgCl2 (2,000 Units)
SKU: E-2013-3
Bioneer Taq DNA Polymerase, 10X Reaction Buffer without MgCl2, 20 mM MgCl2 (2,000 Units)
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Taq DNA polymerase is a thermostable polymerase derived from the gene of Thermus aquaticus YT1 expressed and purified from Escherichia coli .
※This product is shipped in dry ice.
Features and Benefits
Efficiency & Sensitivity
High efficiency and sensitivity to the templates
Optimized buffer delivery
Stable PCR reaction by providing buffers optimized for the enzyme
Reproducibility
Reproducible results with uniform quality products for each batch by manufacturing under the ISO 9001 quality system.
Applications
- Real-time quantification of DNA and cDNA targets using dual probe and dsDNA binding dye
- Gene expression profiling
- Microbial & viral pathogen detection.
Specifications
| 5' to 3' exonuclease activity | Yes |
| 3' to 5' exonuclease activity | No |
| 3' – A overhang | Yes |
| Fragment size | Up to 10 kb |
Kit Content
| Cat. No. | Taq DNA Polymerase (500 U) | 10 x Reaction Buffer | 10 mM dNTPs | 20 mM MgCl2 | Dilution Buffer |
| E-2011 | 100 µl | 1 ml (with MgCl2) | 1 ml | - | 1 ml |
| E-2011-1 | 100 µl | 1 ml (without MgCl2) | 1 ml | 1 ml | 1 ml |
| E-2011-2 | 100 µl | 1 ml (with MgCl2) | - | - | 1 ml |
| E-2011-3 | 100 µl | 1 ml (without MgCl2) | - | 1 ml | 1 ml |
10X reaction buffer with (or without) MgCl2: Tris-HCl, KCl, 15 mM MgCl2, pH 9.0
Dilution buffer: 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50% Glycerol, pH 8.0
10 mM dNTPs mix: 2.5 mM of each dNTP
Concentration
500 units (5 U/μl)
Storage Conditions
20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50% Glycerol pH 8.0.
Storage Temperature
-20°C
Unit Definition
One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72℃.