Bioneer AccuPrep Bacterial RNA Extraction Kit (100 reactions)
SKU: K-3142
Bioneer AccuPrep® Bacterial RNA Extraction Kit (100 reactions)
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AccuPrep® Bacterial RNA Extraction Kit enables rapid and convenient extraction of RNA from gram-positive and gram-negative bacteria. Proteins and other contaminants are effectively removed through silica-based spin columns and optimized buffers. This process is ideal for safe and convenient extraction since no organic solvents are used. Using AccuPrep® Bacterial RNA Extraction Kit, you can extract stable RNA without degradation.
Features and Benefits
- Rapid and convenient extraction of RNA from gram-positive and gram-negative bacteria.
- Complete removal of cell components such as proteins, nucleases and contaminants, resulting in improved efficiency and reproducibility of RT reaction
- Minimized bacterial RNA degradation during extraction process.
- Harmless protocol with no organic solvents used.
- Easy to use because ethanol is added to Washing buffer
Applications
- cDNA synthesis
- RT-PCR
- Quantitative real-time RT-PCR
- Northern blot analysis
Specifications
| Column loading volume | 800 µl |
|---|---|
| Max binding capacity | ~ 120 µg |
| Elution volume | 30 µl - 100 µl |
| Preparation time | ~ 40 min |
| Sample | Amount | Typical yield | Purity |
|---|---|---|---|
| Gram-negative bacteria | 108 cells | 4 - 8 μg | A260/280 > 2.0 A260/230 > 1.7 |
| Gram-positive bacteria | 108 cells | 3 - 6 μg |
Depending on the type of sample, there may be differences in the measured values.
Components
| Components | K-3143 (50 reaction) | K-3142 (100 reaction) |
| RS Buffer (RNA stabilizing) | 40 ml x 1 ea | 80 ml x 1 ea |
| RB Buffer (RNA Binding) | 60 ml x 1 ea | 30 ml x 1 ea 60 ml x 1 ea |
| RWA1 Buffer (1st Washing) | 40 ml x 1 ea | 80 ml x 1 ea |
| RWA2 Buffer (2nd Washing) | 70 ml x 1 ea | 70 ml x 2 ea |
| ER Buffer (Elution) | 10 ml x 1 ea | 20 ml x 1 ea |
| Proteinase K, lyophilized | 25mg x 1 ea | 25mg x 2 ea |
| AccuPrep® Binding Column-Ⅲ | 50 ea | 100 ea |
| 1.5 ml Tube (for collection) | 50 ea | 100 ea |
| User's Guide | 1 ea | 1 ea |