ATTO AB-1850 LumiFl SpectroCapture
SKU: AB-1850
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ATTO AB-1850 LumiFl SpectroCapture
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Purpose and Application:
LumiFLspectrocapture is a highly sensitive spectrophotometer that can measure the spectrum of weak light from bioluminescence or chemiluminescence, which was difficult to measure with existing spectrophotometers using photodiodes or PMT. LumiFLspectrocapture can acquire a whole range of light (ranging from 400-800nm) simultaneously with an ultrasensitive cooled CCD. It can be a powerful tool in designing bioluminescent/chemiluminescent probes in cell imaging study or analysis of mechanisms of bioluminescent/chemiluminescent reaction.
Features:
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An electronic cooled CCD camera is used as a detector.
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A CCD is capable of receiving the light of the entire target wavelength region for measurement at one time and detecting it.
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A CCD has higher quantum efficiency than a photomultiplier tube.
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detection time is shortened with all wavelength simultaneous detection.
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Measurement wavelength range is 400 to 800nm.
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Long-time exposure is available (high sensitive detection of micro specimens)
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When a fluorescent excitation light source is equipped, FRET or fluorescent substance can be measured.
MSE Supplies is an authorized distributor of ATTO products.
Technical Data:
| Model and name | AB-1850 Lumiful Spectro Capture |
|---|---|
| 1. Luminescence fluorescence measurement unit | |
| F-number | F3.0 |
| Measurement wavelength range | 400～900nm |
| Inverse variance | 60nm/mm |
| Wavelength resolution | 1.8nm |
| Reproducibility | ±0.6nm |
| Slit width | 0.01mm~5mm(11 steps) |
| Exposure time | 1/30 sec. ~ 60 min (26 steps) |
| Photosensitive unit | Electronically cooled CCD (-40°C) |
| 2. Spectrometer section | |
| Number of diffraction gratings | 1 (standard) Diffraction grating: 150 lines/mm |
| Blaise wavelength | 500nm |
| 3. Measurement sample section | |
| Measuring vessel | Quartz Cell (10mmx10mm) / 0.2m LPCR Tube / 35mm Dish |
| Sample volume | 10μL～100μL |
| Reagent injection section for instantaneous luminescence | Yes (septum) |
| Excitation light introduction section | FC connector for incident (standard) |
| 4. Software | |
| function | Measurement Control and Spectral Data Analysis |
| interface | USB2.0 |
| 5. Dimensions and Weight | |
| Dimensions & Weight | 510mm(W)×725mm(D)×390mm(H), 58kg |
| power | AC100V, 50/60Hz, 290VA |
Citation and References:
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Y. Mizui, M. Eguchi, M. Tanaka, Y. Ikeda, H. Yoshimura, T. Ozawa, D. Citterio and Y. Hiruta, Long-term single cell bioluminescence imaging with C-3 position protected coelenterazine analogues. Org. Biomol. Chem., 19(3), 579-586 (2021)[PubMed]
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S. Tamaki, N. Kitada, M. Kiyama, R. Fujii, T. Hirano, SB. Kim and S. Maki, Color‑tunable bioluminescence imaging portfolio for cell imaging. Sci. Rep., 11(1), 2219 (2021)[PubMed]
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JW. He, GC. Liu, PX. Dong, ZW. Dong, RP. Zhao, W. Wang and XY. Li, Molecular cloning, characterization, and evolution analysis of the luciferase genes from three sympatric sibling fireflies (Lampyridae: Lampyrinae, Diaphanes). Photocem. Photobiol. Sci., 20(8), 1053-1067 (2021)[PubMed]
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K. Teranishi, Non-invasive and accurate readout of superoxide anion in biological systems by near-infrared light. Anal. Chim. Acta., 1179, 338827 (2021)[PubMed]
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S. Kanie, D. Miura, N. Jimi, T. Hayashi, K. Nakamura, M. Sakata, K. Ogoh, Y. Ohmiya and Y. Mitani, Violet bioluminescent Polycirrus sp. (Annelida: Terebelliformia) discovered in the shallow coastal waters of the Noto Peninsula in Japan. Sci. Rep., 11(1), 19097 (2021)[PubMed]
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E. Belleti, VR. Bevilaqua, AMM. Brito, DA. Modesto, AJC. Lanfredi, VR. Viviani and IL. Nantes‑Cardoso, Synthesis of bioluminescent gold nanoparticle–luciferase hybrid systems for technological applications. Photochem. Photobiol. Sci., 20(11), 1439-1453 (2021)[PubMed]
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Y. Ikeda, M. Orioka, T. Nomoto, Y. Hiruta, N. Nishiyama and D. Citterio, Differential Effect of azetidine substitution in firefly luciferin analogues. Chembiochem, 22(21), 3067-3074 (2021)[PubMed]