ATG5 Monoclonal Antibody
SKU: E-AB-22149-200
ATG5 Monoclonal Antibody
| SKU # | E-AB-22149 |
| Reactivity | Human, Mouse, Rat |
| Host | Mouse |
| Applications | WB, IHC-p |
Product Details
| Isotype | IgG |
| Host | Mouse |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-p |
| Clonality | Monoclonal |
| Immunogen | Recombinant Protein of ATG5 |
| Abbre | ATG5 |
| Synonyms | APG 5, APG 5L, APG5, APG5 autophagy 5 like, APG5 like, APG5-like, APG5L, ASP, ATG 5, ATG5, ATG5 autophagy related 5 homolog, Apoptosis specific protein, Apoptosis-specific protein, Atg5, Autophagy protein 5, Autophagy related 5, Homolog of S Cerevisiae autophagy 5, hAPG5 |
| Swissprot | |
| Observed MW | 55 kDa |
| Cellular Localization | Cytoplasm. Preautophagosomal structure membrane. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. |
| Tissue Specificity | Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Protein A purification |
| Research Areas | Cancer, Cell Biology, Cardiovascular, Metabolism, Signal Transduction |
| Clone No. | 5D3 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-2000 |
| IHC | 1:50-300 |
Background
Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.