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The Tribio® Fast Human IFN- γ ELISA Maxi is a solid phase ELISA designed to measure human IFN- γ levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 3 hours, no need for 5-6 hours (Fig. 1). The detection range is from 6 to 4860 pg/mL. The levels of human IFN- γ samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IFN- γ protein.
Typical Data
This standard curve is provided for demonstration only as Fig.3. A standard curve should be generated for each set of samples assayed.
Sensitivity
The minimum detectable dose (MOD) of human IFN-γ is typically 6 pg/ml.
Specificity
This assay recognizes natural and recombinant human IFN- γ.
For Reseach Use only
Simple Procedures
Simple Standard Preparation
Accurate Standard Curve
Kit Contents & Storage Conditions
| Part | Part# | Description | Storage of Opened/Reconstituted |
|---|---|---|---|
| HumanIFN- γ Microplate | TBS3230UA | 96 well strip microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human IFN- γ. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| HumanIFN- γ Standard | TBS3230UB | 50 µL of Recombinant human IFN- γ protein (145.8ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3230UC | 2.2 mL of HRP-Human IFN- γ antibody. | May be stored for up to 3 months at 2-8 °C. |
| Assay Diluent | TBS3230UD | 12 mL of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8℃. Do not use past kit expiration date.
The kit contains sufficient materials to run an ELISA on one 96 well plate.