Tribioscience Tribo™ Tryptase Activity Assay (TBS2101)
SKU: TBS2101
Tribioscience Tribo™ Tryptase Activity Assay (TBS2101)
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Tryptase, a tetrameric serine proteinase, has emerged as the major component of mast cell granules, comprising up to 20% of the total protein of mast cells derived from lung, colon, and skin tissue. Because it is stored almost exclusively in mast cells, tryptase is a popular indicator of mast cell activation and a target for therapeutic intervention in allergic diseases.
The TribioTM Tryptase Activity Assay Kit provides a quick, efficient, and sensitive system for the evaluation of tryptase activity in cell lysates, supernatants, or for inhibitor screening.
Applications
- Cell culture supernatants, cell lysates, or other tryptase-containing supplies.
- Testing of purified tryptase enzyme, in vitro inhibitor screening.
- Cell degranulation
Key Features
- Sensitive and Accurate: The kit detects as low as 10μM in solution.
- Simple and High-Throughput: The procedure is easily adapted to automation with no separation required as a high-throughput assay.
Storage Conditions:
Store the kit at -20℃, protect from light.
Shelf life: 6 months.
For Research Use only
Standard Preparation (Fig.1)
Fig. 2: pNA Standard Curve
Fig. 3: Tryptase Positive Control Curve
Fig. 4: Effects of pH Value on Tryptase Activity
