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Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a 5´→3´ exonuclease activity. Taq DNA Polymerase is the most common polymerase used for PCR.
Applications
- Regular PCR amplification
- Primer Extension
- DNA Sequencing
Key Features
- Terminal Transferase Activity: Taq DNA Polymerase has terminal transferase activity which results in the addition of a single nucleotide (adenosine) at the 3′ end of the extension product.
- High purity: No contamination activity has been detected in standard test reactions.
Kit Contents (1000 U)
Taq DNA Polymerase: 200µL (5 U/µL)
10x PCR Buffer: 100mM Tris-HCl (pH=8.3), 500mM KCl, 25mM MgCl2, and stabilizer
Storage conditions
The undiluted enzyme solution is stable when stored at -20°C.
Shelf life: 12 months after receipt.