The TribioTM Nucleic Acid Precipitation Enhancer (NAPE) is a highly purified inert carrier to enhance the recovery of nucleic acid (RNA and DNA) in alcohol precipitation. It is a formulated solution of glycogen. Glycogen is insoluble in ethanol or isopropanol and forms a precipitate that traps the nucleic acids in the solution, forming a visible pellet by centrifugation. Therefore, this product is suitable to precipitate RNA and DNA.
Glycogen is a totally inert polysaccharide. It will not interfere with any subsequent reaction; therefore, normally it is not necessary to remove it from nucleic acid preparations. It is different from tRNA, yeast RNA, or sonicated DNA as a carrier, because it is less likely to interfere with downstream applications. Picogram amounts of RNA, DNA, or oligonucleotides can be precipitated using NAPE.
Applications
- Precipitation and purification of RNA from dilute solutions.
- Precipitation and purification of DNA from dilute solutions.
- Recovery of oligonucleotide.