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The Fast Human Complement C4 ELISA is a solid phase ELISA designed to measure human complement C4 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not need 4-5 hours (Fig. 1). The detection range is from 1 to 300 ng/mL. The levels of human complement C4 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human complement C4 protein.Kit Contents and Storage Conditions:
| Reagents | Quantity |
|---|---|
| Human Complement C4 Microplate | 96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human complement C4. |
| Human Complement C4 Standard | 100 µL of Recombinant human complement C4 protein (3 µg/mL). |
| Detection A | 2.1 ml of human Complement C4 antibody-Biotin. |
| Detection B | 100 µL ml of Streptavidin-HRP (100x). |
| Assay Diluent | 20 ml of a buffered protein base with preservatives. |
| 10x WashBuffer | 12ml of concentrated solution(10x). |
| TMB Substrate | 12 ml of ultra-sensitive TMB substrate. |
| Stop Solution | 6ml of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration date.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
For Research Use only
Fig. 1 Simple Assays Procedures
Fig.2 Diagram for Human Complement C4 Standard Preparation
Fig.3 Standard Curve
