This enzyme is useful for enzymatic determination of creatinine, creatine, and sarcosine when coupled with creatinine amidohydrolase and creatine amidinohydrolase.
Tribioscience Sarcosine oxidase, Enzyme Activity (TBP0042)
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Tribioscience Sarcosine oxidase, Enzyme Activity (TBP0042)
Research Use Only. Not intended for human use.
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Preparation and Specification
Appearance: Yellowish amorphous powder, lyophilized
Activity: GradeⅢ 8.0U/mg-solid or more
Contaminant: Catalase ≤1.0%
Stabilizers: Potassium gluconate
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx.43,000 (by SDS-PAGE)
Isoelectric point: 4.8±0.1
Michaelis constant: 2.8×10⁻³M
Inhibitors: Cu⁺⁺, Ag⁺, Hg⁺⁺, p-chloromercuribenzoate, N-ethylmaleimide, SDS
Optimum pH: 7.5-8.5
Optimum temperature: 55-60°C
pH Stability: 6.0-9.5 (25°C, 20hr)
Thermal stability: below 60°C (pH 7.5, 30min)
Applications