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Lactate dehydrogenase (LDH) is a stable cytoplasmic enzyme which is present in most cells. It is released upon damage to the cytoplasmic membrane. The measurement of LDH release is a well-accepted assay to estimate cell membrane integrity and quantify cell cytotoxicity.
The Tribo™ LDH Cytotoxicity Colorimetric Assay Kit uses formazan from oxidized WST-8 to measure the activity of LDH released from damaged cells. The generated signal at OD 460 nm is proportional to the number of lysed cells. The kit provides the easiest and most accurate approach to measure LDH activity and cytotoxicity of a variety of compounds.
Applications
- Cell Proliferation.
- Cytotoxicity and LDH activity in cells and tissue.
Storage Conditions
Store the Reagent at -20°C protected from light. Shelf life: 6 months.
For Research Use only
Fig. 1: LDH Assay Principle
Fig. 2: LDH Standard Curve
