Tribioscience Human Nicotinamide N-Methyltransferase / NNMT Recombinant Protein, Bioactive (TBP0002)

Tribioscience Human Nicotinamide N-Methyltransferase / NNMT Recombinant Protein, Bioactive (TBP0002)

Research Use Only. Not intended for human use.

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NNMT (Nicotinamide N-methyltransferase) belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. It is predominantly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta, and heart. NNMT catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for the biotransformation of many drugs and xenobiotic compounds. Recombinant human NNMT protein, fused to His-tag at N-terminus, was expressed in E. coli, and purified by using chromatography. This enzyme is validated for NNMT activity application.

Product Details

Expression System: E. coli.

Domain: 1-264aa.

UniProt No: P40261.

Alternative Names: Nicotinamide N-methyltransferase/ NNMT.

Product Specification

Molecular Weight: 37.7 kDa (284aa) confirmed by MALDI-TOF

Concentration: 1mg/ml (determined by Bradford assay).

Formulation: Liquid in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, No DTT.

Purity: > 95% by SDS-PAGE.

Tag: His-Tag.

Biological Activity: Specific activity is > 100nmol/min/mg, and is defined as the amount of enzyme that transfers 1.0nmole of methyl group per minute at 37℃.

Storage Condition: Store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.

Applications

• Enzyme Activity, and protein assay by SDS-PAGE