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Tribioscience’s Fast Human Glucagon ELISA is a solid phase ELISA designed to measure glucagon levels in cell culture supernatants. serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to make easy combinations of samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and ultra-sensitive. The measurement can detect a concentration as low as 10 pg/mL, and finish in 2 hours, with no need for 5-6 hours. The detection range is from 10 to 2000 pg/mL. The levels of human glucagon samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human glucagon.
Kit Content and Storage Conditions
| Part | Part# | Description | Storage of Opened/Reconstituted |
|---|---|---|---|
| Human Glucagon Microplate | TBS3244A | 96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human glucagon. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Human Glucagon Standard | TBS3244B | 50 µl of Recombinant human glucagon (50ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3244C | 2.1 ml of HRP- human glucagon antibody. | May be stored for up to 3 months at 2-8 °C. |
| Assay Diluent | TBS3244D | 12 ml of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
For Research Use only
Standard Curve
