Tribioscience Human Adenosylhomocysteinase / AHCY Recombinant Protein, Bioactive (TBP0003)
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Tribioscience Human Adenosylhomocysteinase / AHCY Recombinant Protein, Bioactive (TBP0003)
Research Use Only. Not intended for human use.
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Adenosylhomocysteinase (AHCY) is an enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine (SAH) to adenosine (Ado) and L-homocysteine (Hcy). It regulates the intracellular SAH concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. Recombinant human AHCY protein, fused to His-tag at N-terminus, was expressed in E. coli, and purified by using proprietary chromatography techniques. This enzyme is validated for AHCY activity application.
Product Details
Expression System: E. coli.
Domain: 1-432aa.
UniProt No: P23526.
Alternative Names: Adenosyl homocysteinase, AHCY, AdoHcyase, SAHH, S-adenosylhomocysteine hydrolase.
Product Specification
Molecular Weight: 49.8 kDa (452aa) confirmed by MALDI-TOF
Concentration: 1mg/ml (determined by Bradford assay).
Formulation: Liquid in Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, No DTT.
Purity: > 95% by SDS-PAGE.
Tag: His-Tag.
Biological Activity: Specific activity is > 300pmol/min/mg.
Storage Condition: Store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Applications
- Enzyme Activity, and protein assay by SDS-PAGE