Glycerol is a central component for the synthesis of all lipids and acts as a backbone for triglycerides and phospholipids. It is released from triglycerides as the product of lipolysis. The measurements of circulating glycerol are useful parameters to evaluate lipolysis under various conditions in clinical studies.
Tribioscience’s Glycerol Fluorometric Assay is a robust, simple, and high throughput method based on coupled enzyme reactions. Glycerol is phosphorylated and oxidized to generate hydrogen peroxide, which is catalyzed and reacts with the probe measured by the fluorometric method at Ex/Em = 530/590 nm.
