Tribioscience Glycerol dehydrogenase, Enzyme Activity (TBP0025)

Research Use Only. Not intended for human use.

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Preparation and Specification

Appearance: White amorphous powder, lyophilized

Activity: GradeⅢ 50U/mg-solid or more (containing approx. 50% of stabilizers)

Contaminant: NAD oxidase ≤1.0×10⁻³%

Stabilizer: BSA

Properties

Stability: Stable at -20°C for at least 6 months

Molecular weight: approx. 390,000

Isoelectric point: 4.4±0.1

Michaelis constants: 1.1×10⁻²M (Glycerol), 8.9×10⁻⁵M (NAD⁺)

Structure: 10 subunits (42,000) per enzyme molecule

Inhibitors: p-Chloromercuribenzoate, o-phenanthroline, monoiodoacetate, heavy metal ions (Co⁺⁺, Ni⁺⁺, Cu⁺⁺, Zn⁺⁺, Cd⁺⁺)

Optimum pH: 10.0-10.5

Optimum temperature: 50°C

pH Stability: pH 7.5-10.5 (25°C, 20hr)

Thermal stability: below 55°C (pH 7.5, 15min)

Applications

This enzyme is useful for enzymatic determination of glycerol and of triglyceride when coupled with lipoprotein lipase in clinical analysis.

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