This enzyme is useful for enzymatic determination of NH₃, α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease. This enzyme is also used for enzymatic determination of urea when coupled with urease in clinical analysis.
Tribioscience Glutamate dehydrogenase(NAD dependent), Enzyme Activity (TBP0024)
Regular price
$ 159.95
Couldn't load pickup availability
Still have questions? Ask our experts!
Tribioscience Glutamate dehydrogenase(NAD dependent), Enzyme Activity (TBP0024)
Research Use Only. Not intended for human use.
Buy Tribioscience products from MSE Supplies at the best value.
Preparation and Specification
Appearance: White amorphous powder, lyophilized
Activity: GradeⅡ 100 U/mg-solid or more
Contaminants: NAD oxidase ≤1.0×10⁻²%
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 260,000
Isoelectric point: 5.6
Michaelis constants: 9.21×10⁻³M (NH₃), 4.80×10⁻³M (α-Ketoglutarate)
7.8×10⁻⁵M (L-Glutamate), 1.29×10⁻⁴M (NADH), 5.89×10⁻⁴M (NAD⁺)
Structure: 6 subunits per enzyme molecule
Inhibitors: Heavy metals, PCMB, IAA
Optimum pH: 7.5-8.0 (α-KG→L-Glu) 9.0 (L-Glu→α-KG)
Optimum temperature: 55°C (α-KG→L-Glu) 50°C (L-Glu→α-KG)
pH Stability: pH 5.0-10.0 (25°C, 20hr)
Thermal stability: below 50°C (pH 8.3, 10min)
Applications