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Tribioscience Equine IL2 / Interleukin-2 ELISA Kit

Tribioscience Equine IL2 / Interleukin-2 ELISA Kit

SKU: TBS34002

  • $ 44995
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Tribioscience Equine IL2 / Interleukin-2 ELISA Kit

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Description 

Tribioscience’s Fast Equine IL-2 ELISA is designed to quantitatively detect equine IL-2 levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 0.22 to 160 ng/mL. The levels of equine IL-2 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural equine IL-2 protein.

Typical Data

This standard curve (R2=0.9998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of equine IL-2 is typically 0.2 ng/ml.

The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.

Specificity

This assay recognizes natural and recombinant equine IL-2.

For Research Use Only

Simple Procedures (Fig. 1)

Simple Standard Preparation

Accurate Standard Curve

Kit Content and Storage Conditions

PART PART# DESCRIPTION STORAGE OF OPENED/ RECONSTITUTED
Equine IL-2 Microplate TBS34002A 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for equine IL-2. Return unused wells to the foil pouch. Reseal along entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Equine IL-2 Standard TBS34002B 20 µL of Recombinant equine IL-2 protein (8 μg/mL). Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A TBS34002C 2.2 mL of Biotin-equine IL-2 antibody.  

 

May be stored for up to

3 months at 2-8 °C.*
Detection B TBS34002D 200 µL of Streptavidin-HRP.
Assay Diluent TBS34002E 25 mL of a buffered protein base with preservatives.
Wash Buffer TBS3000W 12 mL of concentrated solution (10x).
TMB Substrate TBS3000T 12 mL of ultra-sensitive TMB substrate.
Stop Solution TBS3000S 6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.