Tribioscience DH10 Competent Cell (10×50 uL), >10^9 (TBS8043)

Tribo™ E. coli DH10 Competent Cells is an E. coli strain DH10B that provides high transformation efficiencies and allows stable replication of high-copy number plasmids. The competent cells can be used in a wide variety of applications for cloning and subcloning. The lacZΔM15 marker provides α-complementation of the β-galactosidase gene, allowing blue/white screening of colonies on plates containing X-gal.

Application

• General cloning and subcloning.
• Blue-white selection.
• Plasmid DNA amplification.
• Plasmid isolation.
• Long-term storage for plasmid stock.
  

Transformation Efficiency (cfu/Ug)
>1×10^⁹

Blue-White Screening
Yes

Reduces Recombination
Yes

Improves Plasmid Quality
Yes

recA Deficient
Yes

Strain
K12

Genotype

F–mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK λ–rpsL(StrR) endA1 nupG

Storage