This enzyme is useful for enzymatic determination of numerous metabolites, e.g. ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol, and of enzyme activities, e.g. GPT, PK, and CPK when coupled with the related enzymes.
Tribioscience D-Lactate dehydrogenase, Enzyme Activity (TBP0031)
SKU: TBP0031-1KU
Tribioscience D-Lactate dehydrogenase, Enzyme Activity (TBP0031)
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Preparation and Specification
Appearance: White amorphos powder, lyophilized
Activity: Grade II 900 U/mg-solid or more
Contaminants: NADH oxidase ≤1.0×10⁻³%
Malate dehydrogenase ≤1.0×10⁻²%
GOT ≤5.0×10⁻³%
GPT ≤5.0×10⁻³%
Myokinase ≤1.0×10⁻²%
Pyruvate kinase ≤1.0×10⁻³%
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 140,000
Isoelectric point: 4.0
Michaelis constant: 6.4×10⁻⁴M (pyruvate, pH 7.0)
Inhibitors: Ag²+, Hg²+, SH-reagents
Optimum pH: 5.0-7.0
Optimum temperature: 30-37°C
pH Stability: pH 5.0-9.0 (25°C, 48hr)
Thermal stability: below 45°C (pH 7.0, 15min)
Applications